INTRAMITOCHONDRIAL LOCALIZATION OF OXIDATIVE ENZYMES IN CULTURED CELLS

Intramitochondrial localization of kynurenine aminotransferase.

Comparisons with the distribution of intramitochondrial marker enzymes have indicated that kynurenine aminotransferase is present in the inner membrane fraction of rat liver mitochondria. It appears to be weakly bound to the inner membrane because it is easily solubilized by increasing the concentration of digitonin. With the use of a-ketoglutarate or oxalacetate as amino group acceptor, the am...

The Cytochemical Localization of Oxidative Enzymes

Methods are presented for the intramitochondrial localization of various diphosphopyridine nucleotide and triphosphopyridine nucleotide-linked dehydrogenases in tissue sections. The cytochemical reactions studied involve the oxidation of the substrates by a specific pyridino-protein. The electron transfer of tetrazolium salt is mediated by the diaphorase system associated with the dehydrogenase...

Cytochemical localization of certain oxidative enzymes.

Employing the technique of cell fractionation, Schneider and Hogeboom (’56) described the localization of certain oxidative enzymes. They reported that cytochrome oxidase and succinic dehydrogenase were found only in the mitochondrial fraction while isocitric dehydrogenase was present in the supernatant and DPN and TPN cytochrome c reductases were observed to be in both fractions. More recently...

Histochemical Localization of Certain Oxidative Enzymes in the Rat Testis.

Localization of kinesin in cultured cells

Kinesin was isolated from bovine brain and used to elicit polyclonal antibodies in rabbits. The specificities of the resulting antibodies were evaluated by immunoblotting. Antibodies purified from these sera by their affinity for brain kinesin react with a polypeptide of approximately 120 kD in extracts from bovine brain, PtK1 cells, and mouse neuroblastoma cells. They bind to a pair of polypep...